Spatial co-transcriptomics reveals discrete stages of the arbuscular mycorrhizal symbiosis.

ABSTRACT: The symbiotic interaction of plants with arbuscular mycorrhizal (AM) fungi is ancient and widespread. Plants provide AM fungi with carbon in exchange for nutrients and water, making this interaction a prime target for crop improvement. However, plant-fungal interactions are restricted to a small subset of root cells, precluding the application of most conventional functional genomic techniques to study the molecular bases of these interactions. Here we used single-nucleus and spatial RNA sequencing to explore both Medicago truncatula and Rhizophagus irregularis transcriptomes in AM symbiosis at cellular and spatial resolution. Integrated, spatially registered single-cell maps revealed infected and uninfected plant root cell types. We observed that cortex cells exhibit distinct transcriptom

SUBMITTER: Serrano K 

PROVIDER: S-EPMC11035146 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature