Crosstalk interactions between transcription factors ERRα and PPARα assist PPARα-mediated gene expression.

ABSTRACT:

Objective

The peroxisome proliferator-activated receptor α (PPARα) is a transcription factor driving target genes involved in fatty acid β-oxidation. To what extent various PPARα interacting proteins may assist its function as a transcription factor is incompletely understood. An ORFeome-wide unbiased mammalian protein-protein interaction trap (MAPPIT) using PPARα as bait revealed a PPARα-ligand-dependent interaction with the orphan nuclear receptor estrogen-related receptor α (ERRα). The goal of this study was to characterize the nature of the interaction in depth and to explore whether it was of physiological relevance.

Methods

We used orthogonal protein-protein interaction assays and pharmacological inhibitors of ERRα in various systems to confirm a functional interaction

SUBMITTER: Desmet SJ 

PROVIDER: S-EPMC11059514 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature