Far-red and sensitive sensor for monitoring real time H₂O₂ dynamics with subcellular resolution and in multi-parametric imaging applications.

ABSTRACT: H₂O₂ is a key oxidant in mammalian biology and a pleiotropic signaling molecule at the physiological level, and its excessive accumulation in conjunction with decreased cellular reduction capacity is often found to be a common pathological marker. Here, we present a red fluorescent Genetically Encoded H₂O₂ Indicator (GEHI) allowing versatile optogenetic dissection of redox biology. Our new GEHI, oROS-HT, is a chemigenetic sensor utilizing a HaloTag and Janelia Fluor (JF) rhodamine dye as fluorescent reporters. We developed oROS-HT through a structure-guided approach aided by classic protein structures and recent protein structure prediction tools. Optimized with JF₆₃₅, oROS-HT is a sensor with 635 nm excitation and 650 nm emission peak

SUBMITTER: Berndt A 

PROVIDER: S-EPMC11065073 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature