A time-resolved single-cell roadmap of the logic driving anterior neural crest diversification from neural border to migration stages.

ABSTRACT: Neural crest cells exemplify cellular diversification from a multipotent progenitor population. However, the full sequence of early molecular choices orchestrating the emergence of neural crest heterogeneity from the embryonic ectoderm remains elusive. Gene-regulatory-networks (GRN) govern early development and cell specification toward definitive neural crest. Here, we combine ultradense single-cell transcriptomes with machine-learning and large-scale transcriptomic and epigenomic experimental validation of selected trajectories, to provide the general principles and highlight specific features of the GRN underlying neural crest fate diversification from induction to early migration stages using Xenopus frog embryos as a model. During gastrulation, a transient neural border zone st

SUBMITTER: Kotov A 

PROVIDER: S-EPMC11087755 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature