Proteogenomic characterization of difficult-to-treat breast cancer with tumor cells enriched through laser microdissection.

ABSTRACT:

Background

Breast cancer (BC) is the most commonly diagnosed cancer and the leading cause of cancer death among women globally. Despite advances, there is considerable variation in clinical outcomes for patients with non-luminal A tumors, classified as difficult-to-treat breast cancers (DTBC). This study aims to delineate the proteogenomic landscape of DTBC tumors compared to luminal A (LumA) tumors.

Methods

We retrospectively collected a total of 117 untreated primary breast tumor specimens, focusing on DTBC subtypes. Breast tumors were processed by laser microdissection (LMD) to enrich tumor cells. DNA, RNA, and protein were simultaneously extracted from each tumor preparation, followed by whole genome sequencing, paired-end RNA sequencing, global proteomics and phosphopro

SUBMITTER: Raj-Kumar PK 

PROVIDER: S-EPMC11094977 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature