CD32 captures committed haemogenic endothelial cells during human embryonic development.

ABSTRACT: During embryonic development, blood cells emerge from specialized endothelial cells, named haemogenic endothelial cells (HECs). As HECs are rare and only transiently found in early developing embryos, it remains difficult to distinguish them from endothelial cells. Here we performed transcriptomic analysis of 28- to 32-day human embryos and observed that the expression of Fc receptor CD32 (FCGR2B) is highly enriched in the endothelial cell population that contains HECs. Functional analyses using human embryonic and human pluripotent stem cell-derived endothelial cells revealed that robust multilineage haematopoietic potential is harboured within CD32⁺ endothelial cells and showed that 90% of CD32⁺ endothelial cells are bona fide HECs. Remarkably, these analyses indica

SUBMITTER: Scarfo R 

PROVIDER: S-EPMC11098737 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature