The mitochondrial tRNA-derived fragment, mt-tRF-Leu^TAA, couples mitochondrial metabolism to insulin secretion.

ABSTRACT:

Objective

The contribution of the mitochondrial electron transfer system to insulin secretion involves more than just energy provision. We identified a small RNA fragment (mt-tRF-Leu^TAA) derived from the cleavage of a mitochondrially-encoded tRNA that is conserved between mice and humans. The role of mitochondrially-encoded tRNA-derived fragments remains unknown. This study aimed to characterize the impact of mt-tRF-Leu^TAA, on mitochondrial metabolism and pancreatic islet functions.

Methods

We used antisense oligonucleotides to reduce mt-tRF-Leu^TAA levels in primary rat and human islet cells, as well as in insulin-secreting cell lines. We performed a joint transcriptome and proteome analysis upon mt-tRF-Leu^TAA inhibition. Additiona

SUBMITTER: Jacovetti C 

PROVIDER: S-EPMC11112368 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature