Sodium/Potassium ATPase Alpha 1 Subunit Fine-tunes Platelet GPCR Signaling Function and is Essential for Thrombosis.

ABSTRACT:

Background

Thrombosis is a major cause of myocardial infarction and ischemic stroke. The sodium/potassium ATPase (NKA), comprising α and β subunits, is crucial in maintaining intracellular sodium and potassium gradients. However, the role of NKA in platelet function and thrombosis remains unclear.

Methods

We utilized wild-type (WT, α1 ^+/+) and NKA α1 heterozygous (α1 ^+/-) mice, aged 8 to 16 weeks, of both sexes. An intravital microscopy-based, FeCl₃-induced carotid artery injury thrombosis model was employed for in vivo thrombosis assessment. Platelet transfusion assays were used to evaluate platelet NKA α1 function on thrombosis. Human platelets isolated from healthy donors and heart failure patients treated with/without digoxi

SUBMITTER: Li OQ 

PROVIDER: S-EPMC11118499 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature