Project description:During the deep reactive ion etching process, the sidewalls of a silicon mold feature rough wavy structures, which can be transferred onto a polydimethylsiloxane (PDMS) microchannel through the soft lithography technique. In this article, we utilized the wavy structures of PDMS microchannel sidewalls to initiate and cavitate bubbles in the presence of acoustic waves. Through bubble cavitation, this acoustofluidic approach demonstrates fast, effective mixing in microfluidics. We characterized its performance by using viscous fluids such as poly(ethylene glycol) (PEG). When two PEG solutions with a resultant viscosity 54.9 times higher than that of water were used, the mixing efficiency was found to be 0.92, indicating excellent, homogeneous mixing. The acoustofluidic micromixer presented here has the advantages of simple fabrication, easy integration, and capability to mix high-viscosity fluids (Reynolds number: ~0.01) in less than 100 ms.
Project description:Bubble dynamics is paramount in comprehending the heat transfer mechanisms of flow boiling in the microchannel within ultrasonic field, which is regarded as a promising method to confront challenges of thermal management posed by microelectronic devices. Nevertheless, the impact of ultrasound on bubble behaviors and its underlying mechanisms remain largely unexplored. This study first delves into the effect of ultrasonic parameters on bubble dynamic behaviors and associated mechanisms, subsequently further analyzing the forces acting on bubbles through the constructed force model. The findings suggest that although growth force serves as the significant resistance, the primary Bjerknes force dominates the rapid detachment of bubbles. The secondary Bjerknes force results in the bubble only sliding along the bottom wall rather than lifting off. Furthermore, the elevated ultrasonic pressure amplitude resulting from augmenting ultrasonic power induces a substantial increase in the critical detachment diameter and growth rate by 55.49 % and 59.42 %, respectively. The enhanced primary Bjerknes force, attributed to the rise in ultrasonic frequency, leads to a 71.42 % increase in sliding velocity and a 46.45 % reduction in growth time. The positive impacts arising from ultrasonic power and frequency are anticipated to notably enhance the thermal performance of microchannels. Besides, surface tension acts as the resistance and diminishes slightly with an augmentation of the boiling number, resulting in a moderate variation in sliding velocity and growth time.
Project description:Microfluidic methods act as an effective motile sperm separation technique used in infertility treatments. This work presents a standalone microfluidic device to separate motile sperm cells from non-motile sperm cells and debris. The separation mechanism is based on the centrifugal force acting on sperms and the ability of progressive motile sperms to swim upstream. The separation of motile sperm is carried out using a simple T-shaped microchannel which constitutes three reservoirs: one inlet and two outlets. Herein, one of the outlets is kept sealed. The sealed channel leads to a high-velocity gradient and a rheotaxis zone at the T junction resulting in the separation of motile sperms. Separated sperms are isolated in a sealed channel with a low Reynolds number flow so that sperms cannot have a net displacement, which ensures that the sperms do not re-enter the fluid flow. CFD simulation is conducted to study the flow fields inside the channel and experimental investigation is carried to observe the separation behaviour of sperms. The reported device provides 100% sperm separation efficiency and ensures the entrapment of sperm cells for a longer period. A modified colorimetric nitroblue tetrazolium test conducted on separated sperm cells shows that there is only a marginal increase in superoxide (O2 -) production, proving normal sperm integrity. This device offers an effective and safe alternative to conventional sperm sorting methods.Supplementary informationThe online version contains supplementary material available at 10.1007/s13534-022-00229-9.
Project description:One of the earliest events in cellular mechanotransduction is often an increase in intracellular calcium concentration associated with intracellular calcium waves (ICWs) in various physiologic or pathophysiologic processes. Although cavitation-induced calcium responses are believed to be important for modulating downstream bioeffects such as cell injury and mechanotransduction in ultrasound therapy, the fundamental mechanisms of these responses have not been elucidated. In this study, we investigated mechanistically the ICWs elicited in single HeLa cells by the tandem bubble-induced jetting flow in a microfluidic system. We identified two distinct (fast and slow) types of ICWs at varying degrees of flow shear stress-induced membrane deformation, as determined by different bubble standoff distances. We showed that ICWs were initiated by an extracellular calcium influx across the cell membrane nearest to the jetting flow, either primarily through poration sites for fast ICWs or opening of mechanosensitive ion channels for slow ICWs, which then propagated in the cytosol via a reaction-diffusion process from the endoplasmic reticulum. The speed of ICW (CICW ) was found to correlate strongly with the severity of cell injury, with CICW in the range of 33 μm/s to 93 μm/s for fast ICWs and 1.4 μm/s to 12 μm/s for slow ICWs. Finally, we demonstrated that micrometer-sized beads attached to the cell membrane integrin could trigger ICWs under mild cavitation conditions without collateral injury. The relation between the characteristics of ICW and cell injury, and potential strategies to mitigate cavitation-induced injury while evoking an intracellular calcium response, may be particularly useful for exploiting ultrasound-stimulated mechanotransduction applications in the future.
Project description:The human Achilles tendon (AT) consists of sub-tendons arising from the gastrocnemius and soleus muscles that exhibit non-uniform tissue displacements thought to facilitate some independent actuation. However, the mechanisms governing non-uniform displacement patterns within the AT, and their relevance to triceps surae muscle contractile dynamics, have remained elusive. We used a dual-probe ultrasound imaging approach to investigate triceps surae muscle dynamics (i.e., medial gastrocnemius-GAS, soleus-SOL) as a determinant of non-uniform tendon tissue displacements in the human AT. We hypothesized that superficial versus deep differences in AT tissue displacements would be accompanied by and correlate with anatomically consistent differences in GAS versus SOL muscle shortening. Nine subjects performed ramped maximum voluntary isometric contractions at each of five ankle joint angles spanning 10° dorsiflexion to 30° plantarflexion. For all conditions, SOL shortened by an average of 78% more than GAS during moment generation. This was accompanied by, on average, 51% more displacement in the deep versus superficial region of the AT. The magnitude of GAS and SOL muscle shortening positively correlated with displacement in their associated sub-tendons within the AT. Moreover, and as hypothesized, superficial versus deep differences in sub-tendon tissue displacements positively correlated with anatomically consistent differences in GAS versus SOL muscle shortening. We present the first in vivo evidence that triceps surae muscle dynamics may precipitate non-uniform displacement patterns in the architecturally complex AT.
Project description:Using archived social media data, the language signatures of people going through breakups were mapped. Text analyses were conducted on 1,027,541 posts from 6,803 Reddit users who had posted about their breakups. The posts include users' Reddit history in the 2 y surrounding their breakups across the various domains of their life, not just posts pertaining to their relationship. Language markers of an impending breakup were evident 3 mo before the event, peaking on the week of the breakup and returning to baseline 6 mo later. Signs included an increase in I-words, we-words, and cognitive processing words (characteristic of depression, collective focus, and the meaning-making process, respectively) and drops in analytic thinking (indicating more personal and informal language). The patterns held even when people were posting to groups unrelated to breakups and other relationship topics. People who posted about their breakup for longer time periods were less well-adjusted a year after their breakup compared to short-term posters. The language patterns seen for breakups replicated for users going through divorce (n = 5,144; 1,109,867 posts) or other types of upheavals (n = 51,357; 11,081,882 posts). The cognitive underpinnings of emotional upheavals are discussed using language as a lens.
Project description:The hourglass is one of the apparatuses familiar to everyone, but reveals intriguing behaviors peculiar to granular materials, and many issues are remained to be explored. In this study, we examined the dynamics of falling sand in a special form of hourglass, i.e., a wedge-shaped hopper, when a suspended granular layer is stabilized to a certain degree. As a result, we found remarkably different dynamic regimes of bubbling and cavity. In the bubbling regime, bubbles of nearly equal size are created in the sand at a regular time interval. In the cavity regime, a cavity grows as sand beads fall before a sudden collapse of the cavity. Bubbling found here is quite visible to a level never discussed in the physics literature and the cavity regime is a novel phase, which is neither continuous, intermittent nor completely blocked phase. We elucidate the physical conditions necessary for the bubbling and cavity regimes and develop simple theories for the regimes to successfully explain the observed phenomena by considering the stability of a suspended granular layer and clogging of granular flow at the outlet of the hopper. The bubbling and cavity regimes could be useful for mixing a fluid with granular materials.
Project description:Spintronics had a widespread impact over the past decades due to transferring information by spin rather than electric currents. Its further development requires miniaturization and reduction of characteristic timescales of spin dynamics combining the sub-nanometre spatial and femtosecond temporal ranges. These demands shift the focus of interest towards the fundamental open question of the interaction of femtosecond spin current (SC) pulses with a ferromagnet (FM). The spatio-temporal properties of the impulsive spin transfer torque exerted by ultrashort SC pulses on the FM open the time domain for probing non-uniform magnetization dynamics. Here we employ laser-generated ultrashort SC pulses for driving ultrafast spin dynamics in FM and analysing its transient local source. Transverse spins injected into FM excite inhomogeneous high-frequency spin dynamics up to 0.6 THz, indicating that the perturbation of the FM magnetization is confined to 2 nm.
Project description:A highly efficient natural light-harvesting antenna has a ring-like structure consisting of dye molecules whose absorption band changes through selective evolutionary processes driven by external stimuli, i.e., sunlight depending on its territory and thermal fluctuations. Inspired by this fact, here, we experimentally and theoretically demonstrate the selective assembling of ring-like arrangements of many silver nanorods with particular shapes and orientations onto a substrate by the light-induced force of doughnut beams with different colours (wavelengths) and polarizations in conjunction with thermal fluctuations at room temperature. Furthermore, the majority of nanorods are electromagnetically coupled to form a prominent red-shifted collective mode of localized surface plasmons resonant with the wavelength of the irradiated light, where a spectral broadening also appears for the efficient broadband optical response. The discovered principle is a promising route for "bio-inspired selective optical assembly" of various nanomaterials that can be used in the wide field of nanotechnology.
Project description:In this paper, thermal mixing characteristics of two miscible fluids in a T-shaped microchannel are investigated theoretically, experimentally, and numerically. Thermal mixing processes in a T-shaped microchannel are divided into two zones, consisting of a T-junction and a mixing channel. An analytical two-dimensional model was first built to describe the heat transfer processes in the mixing channel. In the experiments, de-ionized water was employed as the working fluid. Laser induced fluorescence method was used to measure the fluid temperature field in the microchannel. Different combinations of flow rate ratios were studied to investigate the thermal mixing characteristics in the microchannel. At the T-junction, thermal diffusion is found to be dominant in this area due to the striation in the temperature contours. In the mixing channel, heat transfer processes are found to be controlled by thermal diffusion and convection. Measured temperature profiles at the T-junction and mixing channel are compared with analytical model and numerical simulation, respectively.