Human Rotaviruses of Multiple Genotypes Acquire Conserved VP4 Mutations during Serial Passage.

ABSTRACT: Human rotaviruses exhibit limited tropism and replicate poorly in most cell lines. Attachment protein VP4 is a key rotavirus tropism determinant. Previous studies in which human rotaviruses were adapted to cultured cells identified mutations in VP4. However, most such studies were conducted using only a single human rotavirus genotype. In the current study, we serially passaged 50 human rotavirus clinical specimens representing five of the genotypes most frequently associated with severe human disease, each in triplicate, three to five times in primary monkey kidney cells then ten times in the MA104 monkey kidney cell line. From 13 of the 50 specimens, we obtained 25 rotavirus antigen-positive lineages representing all five genotypes, which tended to replicate more efficiently in MA104 cel

SUBMITTER: Carter MH 

PROVIDER: S-EPMC11209247 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature