Minimal and hybrid hydrogenases are active from archaea.

ABSTRACT: Microbial hydrogen (H₂) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H₂-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H₂. Moreover, we identify and characterize remarkable hybrid complexes formed thr

SUBMITTER: Greening C 

PROVIDER: S-EPMC11216029 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature