High-resolution in situ structures of mammalian respiratory supercomplexes.

ABSTRACT: Mitochondria play a pivotal part in ATP energy production through oxidative phosphorylation, which occurs within the inner membrane through a series of respiratory complexes^1-4. Despite extensive in vitro structural studies, determining the atomic details of their molecular mechanisms in physiological states remains a major challenge, primarily because of loss of the native environment during purification. Here we directly image porcine mitochondria using an in situ cryo-electron microscopy approach. This enables us to determine the structures of various high-order assemblies of respiratory supercomplexes in their native states. We identify four main supercomplex organizations: I₁III₂IV₁, I₁III₂IV₂, I₂I

SUBMITTER: Zheng W 

PROVIDER: S-EPMC11222160 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature