Cadherin-17 as a target for the immunoPET of adenocarcinoma.

ABSTRACT:

Purpose

Cadherin-17 (CDH17) is a calcium-dependent cell adhesion protein that is overexpressed in several adenocarcinomas, including gastric, colorectal, and pancreatic adenocarcinoma. High levels of CDH17 have been linked to metastatic disease and poor prognoses in patients with these malignancies, fueling interest in the protein as a target for diagnostics and therapeutics. Herein, we report the synthesis, in vitro validation, and in vivo evaluation of a CDH17-targeted ⁸⁹Zr-labeled immunoPET probe.

Methods

The CDH17-targeting mAb D2101 was modified with an isothiocyanate-bearing derivative of desferrioxamine (DFO) to produce a chelator-bearing immunoconjugate - DFO-D2101 - and flow cytometry and surface plasmon resonance (SPR) were used to interrogate its antige

SUBMITTER: Delaney S 

PROVIDER: S-EPMC11223962 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature