OTX2 regulates CFTR expression during endoderm differentiation and occupies 3' cis-regulatory elements.

ABSTRACT:

Background

Cell-specific and developmental mechanisms contribute to expression of the cystic fibrosis transmembrane conductance regulator (CFTR) gene; however, its developmental regulation is poorly understood. Here we use human induced pluripotent stem cells differentiated into pseudostratified airway epithelial cells to study these mechanisms.

Results

Changes in gene expression and open chromatin profiles were investigated by RNA-seq and ATAC-seq, and revealed that alterations in CFTR expression are associated with differences in stage-specific open chromatin. Additionally, two novel open chromatin regions, at +19.6 kb and +22.6 kb 3' to the CFTR translational stop signal, were observed in definitive endoderm (DE) cells, prior to an increase in CFTR expression in anterior

SUBMITTER: Kerschner JL 

PROVIDER: S-EPMC11227118 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature