CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures.

ABSTRACT:

Background

The novel endonuclease Cas12b was engineered for targeted genome editing in mammalian cells and is a promising tool for certain applications because of its small size, high sequence specificity and ability to generate relatively large deletions. We previously reported inhibition of the human immunodeficiency virus (HIV) in cell culture infections upon attack of the integrated viral DNA genome by spCas9 and Cas12a.

Methods

We now tested the ability of the Cas12b endonuclease to suppress a spreading HIV infection in cell culture with anti-HIV gRNAs. Virus inhibition was tested in long-term HIV replication studies, which allowed us to test for viral escape and the potential for reaching a CURE of the infected T cells.

Findings

We demonstrate that Cas12b can ac

SUBMITTER: Fan M 

PROVIDER: S-EPMC11228593 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature