High-Efficiency Enrichment by Saturating Nanoliters of Protein Affinity Media.

ABSTRACT: Affinity-purification mass spectrometry (AP-MS) is an established technique for identifying protein-protein interactions (PPIs). The basic technology involves immobilizing a high-specificity ligand to a solid-phase support (e.g., an agarose or magnetic bead) to pull down protein(s) of interest from cell lysates. Although these supports are engineered to minimize interactions with background protein, the conventional method recovers mostly nonspecific binders. The law of mass action for dilute solutions has taught us to use an excess of beads to capture all target proteins, especially weakly interacting ones. However, modern microbead technology presents a binding environment that is much different from a dilute solution. We describe a fluidic platform that captures and processes ultralow n

SUBMITTER: Raval S 

PROVIDER: S-EPMC11234515 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature