Quantification and site-specific analysis of co-occupied N- and O-glycopeptides.

ABSTRACT: Protein glycosylation is a complex post-translational modification that is generally classified as N- or O-linked. Site-specific analysis of glycopeptides is accomplished with a variety of fragmentation methods, depending on the type of glycosylation being investigated and the instrumentation available. For instance, collisional dissociation methods are frequently used for N-glycoproteomic analysis with the assumption that one N-sequon exists per tryptic peptide. Alternatively, electron-based methods are indispensable for O-glycosite localization. However, the presence of simultaneously N- and O-glycosylated peptides could suggest the necessity of electron-based fragmentation methods for N-glycoproteomics, which is not commonly performed. Thus, we quantified the prevalence of N- and O-glyc

SUBMITTER: Chongsaritsinsuk J 

PROVIDER: S-EPMC11245114 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature