Murine Ribonuclease 6 Limits Bacterial Dissemination during Experimental Urinary Tract Infection.

ABSTRACT:

Introduction

The ribonuclease (RNase) A superfamily encodes cationic antimicrobial proteins with potent microbicidal activity toward uropathogenic bacteria. Ribonuclease 6 (RNase6) is an evolutionarily conserved, leukocyte-derived antimicrobial peptide with potent microbicidal activity toward uropathogenic Escherichia coli (UPEC), the most common cause of bacterial urinary tract infections (UTIs). In this study, we generated Rnase6-deficient mice to investigate the hypothesis that endogenous RNase 6 limits host susceptibility to UTI.

Methods

We generated a Rnase6EGFP knock-in allele to identify cellular sources of Rnase6 and determine the consequences of homozygous Rnase6 deletion on antimicrobial activity and UTI susceptibility.

Results

We identified monocytes and ma

SUBMITTER: Cortado H 

PROVIDER: S-EPMC11250601 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature