All-in-One Pseudo-MS³ Method for the Analysis of Gas-Phase Cleavable Protein Crosslinking Reactions.

ABSTRACT: Crosslinking mass spectrometry (XL-MS) supports structure analysis of individual proteins and highly complex whole-cell interactomes. The identification of crosslinked peptides from enzymatic digests remains challenging, especially at the cell level. Empirical methods that use gas-phase cleavable crosslinkers can simplify the identification process by enabling an MS³-based strategy that turns crosslink identification into a simpler problem of detecting two separable peptides. However, the method is limited to select instrument platforms and is challenged by duty cycle constraints. Here, we revisit a pseudo-MS³ concept that incorporates in-source fragmentation, where a fast switch between gentle high-transmission source conditions and harsher in-source fragmentation se

SUBMITTER: Brodie NI 

PROVIDER: S-EPMC11250984 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature