PARK7/DJ-1 deficiency impairs microglial activation in response to LPS-induced inflammation.

ABSTRACT:

Background

Specific microglia responses are thought to contribute to the development and progression of neurodegenerative diseases, including Parkinson's disease (PD). However, the phenotypic acquisition of microglial cells and their role during the underlying neuroinflammatory processes remain largely elusive. Here, according to the multiple-hit hypothesis, which stipulates that PD etiology is determined by a combination of genetics and various environmental risk factors, we investigate microglial transcriptional programs and morphological adaptations under PARK7/DJ-1 deficiency, a genetic cause of PD, during lipopolysaccharide (LPS)-induced inflammation.

Methods

Using a combination of single-cell RNA-sequencing, bulk RNA-sequencing, multicolor flow cytometry and immunofluo

SUBMITTER: Lind-Holm Mogensen F 

PROVIDER: S-EPMC11253405 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature