NDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment.

ABSTRACT:

Introduction

Glioma is the most frequent and lethal form of primary brain tumor. The molecular mechanism of oncogenesis and progression of glioma still remains unclear, rendering the therapeutic effect of conventional radiotherapy, chemotherapy, and surgical resection insufficient. In this study, we sought to explore the function of HEC1 (highly expressed in cancer 1) in glioma; a component of the NDC80 complex in glioma is crucial in the regulation of kinetochore.

Methods

Bulk RNA and scRNA-seq analyses were used to infer HEC1 function, and in vitro experiments validated its function.

Results

HEC1 overexpression was observed in glioma and was indicative of poor prognosis and malignant clinical features, which was confirmed in human glioma tissues. High HEC1 expressio

SUBMITTER: Ye W 

PROVIDER: S-EPMC11255415 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature