Distance tuneable integral membrane protein containing floating bilayers via in situ directed self-assembly.

ABSTRACT: Model membranes allow for structural and biophysical studies on membrane biochemistry at the molecular level, albeit on systems of reduced complexity which can limit biological accuracy. Floating supported bilayers offer a means of producing planar lipid membrane models not adhered to a surface, which allows for improved accuracy compared to other model membranes. Here we communicate the incorporation of an integral membrane protein complex, the multidomain β-barrel assembly machinery (Bam), into our recently developed in situ self-assembled floating supported bilayers. Using neutron reflectometry and quartz crystal microbalance measurements we show this sample system can be fabricated using a two-step self-assembly process. We then demonstrate the complexity of the model membrane a

SUBMITTER: Hall SCL 

PROVIDER: S-EPMC11256219 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature