Nanosecond Transient IR Spectroscopy of Halorhodopsin in Living Cells.

ABSTRACT: The ability to track minute changes of a single amino acid residue in a cellular environment is causing a paradigm shift in the attempt to fully understand the responses of biomolecules that are highly sensitive to their environment. Detecting early protein dynamics in living cells is crucial to understanding their mechanisms, such as those of photosynthetic proteins. Here, we elucidate the light response of the microbial chloride pump NmHR from the marine bacterium Nonlabens marinus, located in the membrane of living Escherichia coli cells, using nanosecond time-resolved UV/vis and IR absorption spectroscopy over the time range from nanoseconds to seconds. Transient structural changes of the retinal cofactor and the surrounding apoprotein are recorded using light-indu

SUBMITTER: Oldemeyer S 

PROVIDER: S-EPMC11258790 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature