Blue valorization of lignin-derived monomers via reprogramming marine bacterium Roseovarius nubinhibens.

ABSTRACT: Biological valorization of lignin, the second most abundant biopolymer on Earth, is an indispensable sector to build a circular economy and net-zero future. However, lignin is recalcitrant to bioupcycling, demanding innovative solutions. We report here the biological valorization of lignin-derived aromatic carbon to value-added chemicals without requesting extra organic carbon and freshwater via reprogramming the marine Roseobacter clade bacterium Roseovarius nubinhibens. We discovered the unusual advantages of this strain for the oxidation of lignin monomers and implemented a CRISPR interference (CRISPRi) system with the lacI-P_trc inducible module, nuclease-deactivated Cas9, and programmable gRNAs. This is the first CRISPR-based regulatory system in R. n

SUBMITTER: Wei Y 

PROVIDER: S-EPMC11267941 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature