Placental cell conditioned media modifies hematopoietic stem cell transcriptome invitro.

ABSTRACT:

Introduction

Hematopoietic stem cells are cells that differentiate into blood cell types. Although the placenta secretes hormones, proteins and other factors important for maternal/fetal health, cross-talk between placental and hematopoietic stem cells is poorly understood. Moreover, toxicant impacts on placental-hematopoietic stem cell communication is understudied. The goals of this study were to determine if factors secreted from placental cells alter transcriptomic responses in hematopoietic stem cells and if monoethylhexyl phthalate (MEHP), the bioactive metabolite of the pollutant diethylhexyl phthalate, modifies these effects.

Methods

We used K-562 and BeWo cells as in vitro models of hematopoietic stem cells and placental syncytiotrophoblasts, respectively. We treate

SUBMITTER: Harris SM 

PROVIDER: S-EPMC11270901 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature