Clostridium septicum manifests a bile salt germinant response mediated by Clostridioides difficile csp gene orthologs.

ABSTRACT: Clostridium septicum infections are highly predictive of certain malignancies in human patients. To initiate infections, C. septicum spores must first germinate and regain vegetative growth. Yet, what triggers the germination of C. septicum spores is still unknown. Here, we observe that C. septicum germinates in response to specific bile salts. Putative bile salt recognition genes are identified in C. septicum based on their similarity in sequence and organization to bile salt-responsive csp genes in Clostridioides difficile. Inactivating two of these csp orthologs (cspC-82 and cspC-1718) results in mutant spores that no longer germinate in the presence of their respective cognate bile salts. Additionally, inactivating the putative cspBA or sleC genes in C. septicum abrogates the germinati

SUBMITTER: Sum R 

PROVIDER: S-EPMC11300598 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature