DF-1-Derived exosomes mediate transmission of reticuloendotheliosis virus and resist REV-specific antibodies.

ABSTRACT:

Background

Reticuloendotheliosis virus (REV), a member of the family Retroviridae, is a hot area of research, and a previous study showed that exosomes purified from REV-positive semen were not blocked by REV-specific neutralizing antibodies and established productive infections.

Methods

To further verify the infectivity of exosomes from REV-infected cells, we isolated and purified exosomes from REV-infected DF-1 cells and identified them using Western blot and a transmission electron microscope. We then inoculated 7-day-old embryonated eggs, 1-day-old chicks and 23-week-old hens with and without antibody treatment. REV was administered simultaneously as a control.

Results

In the absence of antibodies, the results indicated that REV-exosomes and REV could infect chick

SUBMITTER: Wang Z 

PROVIDER: S-EPMC11304787 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature