Redundancy of p75NTR neurotrophin receptor function in development, growth and fertility in the rat.

ABSTRACT: The p75NTR neurotrophin receptor has positive and negative roles regulating cell survival in the nervous system. Unambiguous interpretation of p75NTR function in vivo has been complicated, however, by residual expression of alternate forms of p75NTR protein in initial p75NTR knock-out mouse models. As rats are the preferred rodent for studying brain and behaviour, and to simplify interpretation of the knock-out phenotype, we report here the generation of a mutant rat devoid of the p75NTR protein. TALEN-mediated recombination in embryonic stem cells (ESCs) was used to flank exon 2 of p75NTR with Lox P sites and produce transgenic rats carrying either un-recombined floxed p75NTR^Ex2-fl, or recombined, exon-2 deleted p75NTR^Ex2-Δ alleles. Crossing p75NTR^Ex2-fl r

SUBMITTER: Meek S 

PROVIDER: S-EPMC11319401 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature