An in vitro 3-dimensional Collagen-based Corneal Construct with Innervation Using Human Corneal Cell Lines.

ABSTRACT:

Purpose

To develop a 3-dimensional corneal construct suitable for in vitro studies of disease conditions and therapies.

Design

In vitro human corneal constructs were created using chemically crosslinked collagen and chondroitin sulfate extracellular matrix and seeded with 3 human corneal cell types (epithelial, stromal, and endothelial) together with neural cells. The neural cells were derived from hybrid neuroblastoma cells and the other cells used from immortalized human corneal cell lines. To check the feasibility and characterize the constructs, cytotoxicity, cell proliferation, histology, and protein expression studies were performed.

Results

Optimized culture condition permitted synchronized viability across the cell types within the construct. The construct sho

SUBMITTER: Islam MM 

PROVIDER: S-EPMC11321308 | biostudies-literature | 2024 Nov-Dec

REPOSITORIES: biostudies-literature