Evaluation of a one-step sample preparation protocol for analysis of total serum testosterone by LC-MS/MS.

ABSTRACT: In this study, a one-step method for liquid-liquid extraction has been compared against a two-step procedure for testosterone assays in terms of accuracy, specificity, recovery, lipid removal and baseline noise, using QCs and unknown samples. The difference in accuracy was less than 5% for adult sera, while it was less than 10% for prepubescent sera. To compare specificity, the ion ratio transition of 289 → 97 to 289 → 109 was monitored for all QCs and unknown samples; no interference in the testosterone peak was observed for any tested sample prepared by either the one-step or two-step procedure. The baseline comparison of LC-MS/MS chromatograms of samples indicated that samples prepared by the one-step procedure were of the same quality as those prepared by the two-step procedure; howeve

SUBMITTER: Ke Y 

PROVIDER: S-EPMC11322748 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature