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Requirement of Pdgfrα+ cells for calvarial bone repair.

ABSTRACT: Platelet-derived growth factor receptor α (PDGFRα) is often considered as a general marker of mesenchymal cells and fibroblasts, but also shows expression in a portion of osteoprogenitor cells. Within the skeleton, Pdgfrα+ mesenchymal cells have been identified in bone marrow and periosteum of long bones, where they play a crucial role in participating in fracture repair. A similar examination of Pdgfrα+ cells in calvarial bone healing has not been examined. Here, we utilize Pdgfrα-CreERTM;mT/mG reporter animals to examine the contribution of Pdgfrα+ mesenchymal cells to calvarial bone repair through histology and single-cell RNA sequencing (scRNA-Seq). Results showed that Pdgfrα+ mesenchymal cells are present in several cell clusters by scRNA-Seq, and by histology a dramatic increase in Pdgfrα+ cells populated the defect site at early timepoints to give rise to healed bone tissue overtime. Notably, diphtheria toxin-mediated ablation of Pdgfrα reporter+ cells resulted in significantly impaired calvarial bone healing. Our findings suggest that Pdgfrα-expressing cells within the calvarial niche play a critical role in the process of calvarial bone repair.

SUBMITTER: Xing X

PROVIDER: S-EPMC11328938 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Requirement of Pdgfrα+ cells for calvarial bone repair.

Xing Xin X Li Zhao Z Xu Jiajia J Chen Austin Z AZ Archer Mary M Wang Yiyun Y Xu Mingxin M Wang Ziyi Z Zhu Manyu M Qin Qizhi Q Thottappillil Neelima N Zhou Myles M James Aaron W AW

Stem cells translational medicine 20240801 8

Platelet-derived growth factor receptor α (PDGFRα) is often considered as a general marker of mesenchymal cells and fibroblasts, but also shows expression in a portion of osteoprogenitor cells. Within the skeleton, Pdgfrα+ mesenchymal cells have been identified in bone marrow and periosteum of long bones, where they play a crucial role in participating in fracture repair. A similar examination of Pdgfrα+ cells in calvarial bone healing has not been examined. Here, we utilize Pdgfrα-CreERTM;mT/mG ...[more]

PMID: 38986535

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Requirement of Pdgfrα+ cells for calvarial bone repair.

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Requirement of Pdgfrα+ cells for calvarial bone repair.

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