Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector.

ABSTRACT: The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p < 0.001) than other PP. The use of the conical-PP can save research budgets in the current inflationary environment.

SUBMITTER: Marquez M 

PROVIDER: S-EPMC11330712 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature