Revealing the Mechanism of Esculin in Treating Renal Cell Carcinoma Based on Network Pharmacology and Experimental Validation.

ABSTRACT:

Purpose

This study aims to explore the potential mechanisms of esculin in the treatment of renal cell carcinoma (RCC).

Methods

We employed network pharmacology to predict the potential mechanisms and targets of esculin in RCC. Molecular docking techniques were then employed to validate the predicted targets. Additionally, a series of in vitro experiments were conducted to verify the anticancer effects of esculin on RCC cells, including the CCK-8 assay, EdU assay, wound healing assay, apoptosis assay, and Western blot.

Results

Network pharmacology and molecular docking results identified GAPDH, TNF, GSK3B, CCND1, MCL1, IL2, and CDK2 as core targets. GO and KEGG analyses suggested that esculin may influence apoptotic processes and target the PI3K/Akt pathway in RCC. Fur

SUBMITTER: Chen Z 

PROVIDER: S-EPMC11352311 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature