Virtual Screening and Validation of Affinity DNA Functional Ligands for IgG Fc Segment.

ABSTRACT: The effective attachment of antibodies to the immune sensing interface is a crucial factor that determines the detection performance of immunosensors. Therefore, this study aims to investigate a novel antibody immobilization material with low molecular weight, high stability, and excellent directional immobilization effect. In this study, we employed molecular docking technology based on the ZDOCK algorithm to virtually screen DNA functional ligands (DNAFL) for the Fc segment of antibodies. Through a comprehensive analysis of the key binding sites and contact propensities at the interface between DNAFL and IgG antibody, we have gained valuable insights into the affinity relationship, as well as the principles governing amino acid and nucleotide interactions at this interface. Furthermore,

SUBMITTER: Yang Q 

PROVIDER: S-EPMC11354668 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature