The Freshwater Cyanobacterium Synechococcus elongatus PCC 7942 Does Not Require an Active External Carbonic Anhydrase.

ABSTRACT: Under standard laboratory conditions, Synechococcus elongatus PCC 7942 lacks EcaA^Syn, a periplasmic carbonic anhydrase (CA). In this study, a S. elongatus transformant was created that expressed the homologous EcaA^Cya from Cyanothece sp. ATCC 51142. This additional external CA had no discernible effect on the adaptive responses and physiology of cells exposed to changes similar to those found in S. elongatus natural habitats, such as fluctuating CO₂ and HCO₃^- concentrations and ratios, oxidative or light stress, and high CO₂. The transformant had a disadvantage over wild-type cells under certain conditions (Na⁺ depletion, a reduction in CO₂). S. elongatus cells lacked

SUBMITTER: Kupriyanova EV 

PROVIDER: S-EPMC11360081 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature