Dataset Information

Spatially structured exchange of metabolites enhances bacterial survival and resilience in biofilms.

ABSTRACT: Biofilm formation enhances bacterial survival and antibiotic tolerance, but the underlying mechanisms are incompletely understood. Here, we show that biofilm growth is accompanied by a reduction in bacterial energy metabolism and membrane potential, together with metabolic exchanges between the inner and outer regions in biofilms. More specifically, nutrient-starved cells in the interior supply amino acids to cells in the periphery, while peripheral cells experience a decrease in membrane potential and provide fatty acids to interior cells. Fatty acids facilitate the repair of starvation-induced membrane damage in inner cells and enhance their survival in the presence of antibiotics. Thus, metabolic exchanges between inner and outer cells contribute to survival of the nutrient-starved inner cells and contribute to antibiotic tolerance within the biofilm.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC11366000 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Spatially structured exchange of metabolites enhances bacterial survival and resilience in biofilms.

Zhang Yuzhen Y Cai Yukmi Y Zhang Bing B Zhang Yi-Heng P Job YPJ

Nature communications 20240831 1

Biofilm formation enhances bacterial survival and antibiotic tolerance, but the underlying mechanisms are incompletely understood. Here, we show that biofilm growth is accompanied by a reduction in bacterial energy metabolism and membrane potential, together with metabolic exchanges between the inner and outer regions in biofilms. More specifically, nutrient-starved cells in the interior supply amino acids to cells in the periphery, while peripheral cells experience a decrease in membrane potent ...[more]

PMID: 39217184

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Dataset Information

Spatially structured exchange of metabolites enhances bacterial survival and resilience in biofilms.

Publications

Spatially structured exchange of metabolites enhances bacterial survival and resilience in biofilms.

Similar Datasets

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