Evaluating DNA Methylation in Random Fine Needle Aspirates from the Breast to Inform Cancer Risk.

ABSTRACT:

Introduction

Critical regulatory genes are functionally silenced by DNA hypermethylation in breast cancer and premalignant lesions. The objective of this study was to examine whether DNA methylation assessed in random fine needle aspirates (rFNA) can be used to inform breast cancer risk.

Methods

In 20 women with invasive breast cancer scheduled for surgery at Johns Hopkins Hospital, cumulative methylation status was assessed in a comprehensive manner. rFNA was performed on tumors, adjacent normal tissues, and all remaining quadrants. Pathology review was conducted on blocks from all excised tissue. The cumulative methylation index (CMI) for 12 genes was assessed by a highly sensitive QM-MSP assay in 280 aspirates and tissue from 11 incidental premalignant lesions. Mann-Whitn

SUBMITTER: Visvanathan K 

PROVIDER: S-EPMC11401740 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature