Mechanically sheared axially swept light-sheet microscopy.

ABSTRACT: We present a mechanically sheared image acquisition format for upright and open-top light-sheet microscopes that automatically places data in its proper spatial context. This approach, which reduces computational post-processing and eliminates unnecessary interpolation or duplication of the data, is demonstrated on an upright variant of axially swept light-sheet microscopy (ASLM) that achieves a field of view, measuring 774 × 435 microns, that is 3.2-fold larger than previous models and a raw and isotropic resolution of ∼460 nm. Combined, we demonstrate the power of this approach by imaging sub-diffraction beads, cleared biological tissues, and expanded specimens.

SUBMITTER: Lin J 

PROVIDER: S-EPMC11407235 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature