NAT10 resolves harmful nucleolar R-loops depending on its helicase domain and acetylation of DDX21.

ABSTRACT:

Background

Aberrant accumulation of R-loops leads to DNA damage, genome instability and even cell death. Therefore, the timely removal of harmful R-loops is essential for the maintenance of genome integrity. Nucleolar R-loops occupy up to 50% of cellular R-loops due to the frequent activation of Pol I transcription. However, the mechanisms involved in the nucleolar R-loop resolution remain elusive. The nucleolar acetyltransferase NAT10 harbors a putative RecD helicase domain (RHD), however, if NAT10 acts in the R-loop resolution is still unknown.

Methods

NAT10 knockdown cell lines were constructed using CRISPR/Cas9 technology and short hairpin RNA targeting NAT10 mRNA, respectively. The level of R-loops was detected by immunofluorescent staining combined with RNase H treatme

SUBMITTER: Su K 

PROVIDER: S-EPMC11468200 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature