Mapping multiple RNA modifications simultaneously by proximity barcode sequencing.

ABSTRACT: RNA is subject to a multitude of different chemical modifications that collectively represent the epitranscriptome. Individual RNA modifications including N6-methyladenosine (m⁶A) on mRNA play essential roles in the posttranscriptional control of gene expression. Recent technological advances have enabled the transcriptome-wide mapping of certain RNA modifications, to reveal their broad relevance and characteristic distribution patterns. However, convenient methods that enable the simultaneous mapping of multiple different RNA marks within the same sample are generally lacking. Here we present EpiPlex RNA modification profiling, a bead-based proximity barcoding assay with sequencing readout that expands the scope of molecular recognition-based RNA modification detection to multi

SUBMITTER: Sendinc E 

PROVIDER: S-EPMC11482933 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature