Efficient and easy-to-use capturing three-dimensional metagenome interactions with GutHi-C.

ABSTRACT: Hi-C can obtain three-dimensional chromatin structure information and is widely used for genome assembly. We constructed the GutHi-C technology. As shown in the graphical abstract, it is a highly efficient and quick-to-operate method and can be widely used for human, livestock, and poultry gut microorganisms. It provides a reference for the Hi-C methodology of the microbial metagenome. DPBS, Dulbecco's phosphate-buffered saline; Hi-C, high-through chromatin conformation capture; LB, Luria-Bertani; NGS, next-generation sequencing; PCR, polymerase chain reaction; QC, quality control.

SUBMITTER: Lu YX 

PROVIDER: S-EPMC11487548 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature