Waveform Optimization for the <i>In Vitro</i> Detection of Caffeic Acid by Fast-Scan Cyclic Voltammetry.

ABSTRACT: Caffeic acid is a polyphenol of critical importance in plants, involved in a variety of physiological processes including lignin formation, cellular growth, stress response, and external signaling. This small molecule also acts as a powerful antioxidant and thus has therapeutic potential for a variety of health conditions. Traditional methods of detecting caffeic acid lack appropriate temporal resolution to monitor real time concentration changes on a subsecond time scale with nM detection limits. Here we report on the first usage of fast-scan cyclic voltammetry with carbon fiber microelectrodes for the detection of caffeic acid. Through the use of flow injection analysis, the optimal waveform for its detection under acidic conditions at a scan rate of 400 V/s was determined to be sawtooth

SUBMITTER: Tonn JN 

PROVIDER: S-EPMC11487675 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature