Circular RNA landscape in extracellular vesicles from human biofluids.

ABSTRACT:

Background

Circular RNAs (circRNAs) have emerged as a prominent class of covalently closed single-stranded RNA molecules that exhibit tissue-specific expression and potential as biomarkers in extracellular vesicles (EVs) derived from liquid biopsies. Still, their characteristics and applications in EVs remain to be unveiled.

Methods

We performed a comprehensive analysis of EV-derived circRNAs (EV-circRNAs) using transcriptomics data obtained from 1082 human body fluids, including plasma, urine, cerebrospinal fluid (CSF), and bile. Our validation strategy utilized RT-qPCR and RNA immunoprecipitation assays, complemented by computational techniques for analyzing EV-circRNA features and RNA-binding protein interactions.

Results

We identified 136,327 EV-circRNAs from vari

SUBMITTER: Zhao J 

PROVIDER: S-EPMC11526565 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature