Streptococcus canis transcriptomic modifications in host cell entry environments of human keratinocytes.

ABSTRACT:

Background

Streptococcus canis is a commensal bacterium in companion animals. This microorganism can infect humans who have been in deep contact with or bitten by pet dogs, suggesting that the skin/soft tissue is one of infection entry sites. To understand pathological process in human cells, we aimed to determine S. canis transcriptomic changes in invasive environments of human keratinocytes.

Methods

We selected one isolate from candidates with whole-genome sequences, based on re-obtained cell invasion ability (CIA) data into human keratinocytes along with bacterial cytotoxicity. RNA-sequencing was conducted for the samples at baselines and 2 h/5 hr post-inoculation using NovaSeq 6000. Global/differential gene expression analyses [principal component analysis (PCA)/k-means

SUBMITTER: Yoshida H 

PROVIDER: S-EPMC11533360 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature