Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.

ABSTRACT:

Background

Liver organoid serves as an alternative model for liver pathophysiology in carbohydrate or lipid metabolism and xenobiotic metabolism transformation. Biomechanical cues including spaceflight mission can affect liver organoid construction and their related functions, but their underlying mechanisms are not fully understood yet. Here, a rotating cell culture device, namely Rotating Flat Chamber (RFC), was specifically designed for adhering cells or cell aggregated to elucidate the effects of altered gravity vector on HepaRG-derived liver organoids construction.

Results

The organoids so formed under RFC presented the fast growth rate and large projection area. Meanwhile, the expressions of two pluripotency markers of SOX9 and CD44 were enhanced. This finding was posi

SUBMITTER: Zhong S 

PROVIDER: S-EPMC11568593 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature