Investigating the Potential of Multilineage Differentiating Stress-Enduring Cells for Osteochondral Healing.

ABSTRACT:

Objective

Multilineage differentiating stress-enduring (Muse) cells, a pluripotent stem cell subset of mesenchymal stem cells (MSCs), have shown promise for various tissue repairs due to their stress tolerance and multipotent capabilities. We aimed to investigate the differentiation potential in vitro, the dynamics in vivo, and the reparative contribution of Muse cells to osteochondral lesions.

Design

Labeled MSCs were cultured and sorted into Muse and non-Muse (MSCs without Muse cells) groups. These cells were then formed into spheroids, and chondrogenic differentiation was assessed in vitro. Twenty-one immunocompromised mice were used as the in vivo models of osteochondral lesions. Live imaging, macroscopic evaluation, and histological and immun

SUBMITTER: Watanabe S 

PROVIDER: S-EPMC11569652 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature