A Comprehensive LC-MS Metabolomics Assay for Quantitative Analysis of Serum and Plasma.

ABSTRACT: Background/Objectives: Targeted metabolomics is often criticized for the limited metabolite coverage that it offers. Indeed, most targeted assays developed or used by researchers measure fewer than 200 metabolites. In an effort to both expand the coverage and improve the accuracy of metabolite quantification in targeted metabolomics, we decided to develop a comprehensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay that could quantitatively measure more than 700 metabolites in serum or plasma. Methods: The developed assay makes use of chemical derivatization followed by reverse phase LC-MS/MS and/or direct flow injection MS (DFI-MS) in both positive and negative ionization modes to separate metabolites. Multiple reaction monitoring (MRM), in combination wit

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11596266 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature