Development of an RNA virus vector for non-transgenic genome editing in tobacco and generation of <i>berberine bridge enzyme-like</i> mutants with reduced nicotine content.

ABSTRACT: Tobacco (Nicotiana tabacum) plants synthesize the psychoactive pyridine alkaloid nicotine, which has sparked growing interest in reducing nicotine levels through genome editing aiming at inactivating key biosynthetic genes. Although stable transformation-mediated genome editing is effective in tobacco, its polyploid nature complicates the complete knockout of genes and the segregation of transgenes from edited plants. In this study, we developed a non-transgenic genome editing method in tobacco by delivering the CRISPR/Cas machinery via an engineered negative-strand RNA rhabdovirus vector, followed by the regeneration of mutant plants through tissue culture. Using this method, we targeted six berberine bridge enzyme-like protein (BBL) family genes for mutagenesis, whic

SUBMITTER: Xiang H 

PROVIDER: S-EPMC11624166 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature