A Novel Normalized Quantitative Real-Time PCR Approach for Ensuring Roe Deer (Capreolus capreolus) Meat Authenticity in Game Meat Foods.

ABSTRACT: Roe deer meat is a prized game product in many European countries. However, concerns exist regarding the accuracy of the amount of declared roe deer in processed game meat foods. This study aimed to develop a reliable method for the detection and quantification of roe deer in commercialized game meat products. A TaqMan probe-based quantitative real-time PCR (qPCR) assay was designed, targeting a single-copy 120-bp region of the roe deer agouti signaling protein (ASIP) encoding gene. The method employed the normalized ∆Cq approach to establish a calibration curve for roe deer detection and quantification within 0.05-50% (w/w) in complex raw and processed matrices. The method proved to be specific for roe deer identification, achieving limits of detection and quantification of

SUBMITTER: Adenuga BM 

PROVIDER: S-EPMC11640146 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature