Exploring cytokine outputs for <i>ex vivo</i> diagnostics in drug reaction with eosinophilia and systemic symptoms (DRESS).

ABSTRACT:

Background

In an exploratory study to assess the potential to individualize T-cell diagnostics in antibiotic-associated severe T-cell mediated hypersensitivity, we focused on drug reaction with eosinophilia and systemic symptoms (DRESS) and the related cytokine outputs IL-4 and IL-5.

Methods

Patients with well-phenotyped RegiSCAR ≥4 DRESS, positive intradermal skin testing, and a previous negative IFN-γ Enzyme-Linked ImmunoSpot (ELISpot) assay were prospectively recruited. We specifically performed an ELISpot assay with IL-4 and IL-5 cytokine outputs. As comparative controls, these cytokine outputs were performed simultaneously in patients with a positive ex vivo IFN-γ release ELISpot result.

Results

Four antibiotic-associated DRESS cases were included. The IL-

SUBMITTER: Copaescu AM 

PROVIDER: S-EPMC11665396 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature